App Notes

High Throughput Analysis of Cannabinoids by LC-UV

October 9, 2019

As the cannabis market grows, interest in more detailed analysis of cannabinoid profiles is expanding because more comprehensive data can be used for strain identification as well as to ensure more accurate potency testing. The LC-UV method shown here uses a Raptor ARC-18 column to fully resolve 16 major and most frequently observed minor cannabinoids for which commercial standards are available.

Avoiding Contamination in Cannabis Quality Control Sample Preparation

October 4, 2019

Patient and consumer safety is a critical factor during manufacturing of your products. In addition, false test results in product quality testing can lead to unnecessary scrapping of batches and product recalls. Good pipetting practices using filtered and pure pipette tips help ensure contamination-free pipetting to prevent contamination during sample preparation, ensuring true test results and patient and consumer safety

Application Note: How to Avoid Contamination in Pipetting

September 25, 2019

Preventing contamination in pipetting is paramount to achieving reliable results. It requires identification of the potential contamination mechanism in order that they can all be addressed. This paper addresses the three contamination types that originate from pipetting: Pipette-to-sample contamination, sample-to-pipette contamination, and sample-to-sample contamination. Keep in  mind: If your testing procedure in your Cannabis environment  requires you to repeatedly portion out exact volumes of liquids involved, our bottle-top dispensers will give you a steady hand to ensure accurate and consistent results.

Application Note: Preparation of Standards - Easier than ever

September 25, 2019

Improving your HPLC Sample Preparation is easier than ever with the Sartorius Cubis® MSA Dosing System. The Cubis MSA individual system with Q-App software automatically corrects the solvent volume to the appropriate value, eliminating the need to reach the precise target weight. At the end of the procedure, the weight of the compound(s) and solvents is used to calculate the verified concentration(s) and the verified volume of the standard prepared.

Application Note: Recovery & Adsorption of Therapeutic Proteins

September 25, 2019

We tested the novel filter device Claristep® for the preparation of protein samples containing one of four different target molecules: an anti-malaria vaccine candidate, aviscuminum, interferon alfa-2B, or monoclonal antibody (mAb) P2G12. In comparison to the standard protocols for sample preparation prior to analysis by either reversed-phase or SEC HPLC, the Claristep® filter did not exhibit a significant difference in protein concentration if the target molecules were present with at least 1.0 g L-1. A significantly reduced product concentration was observed in the Claristep® filtered samples compared to the standard protocol for mAb P2G12 if the concentrations were lower. The protein adsorption to the filter material was well described by a Langmuir adsorption isotherm. Additionally, the Claristep® filters facilitated a rapid sample preparation with minimal volume losses.

Poster: Analysis of Aqueous Mobile Phase Sample Prep & Filtration

September 25, 2019

The two-fold objective of this study was to 1) demonstrate that aqueous mobile phase preparations from purchased lab grade bottled water yielded comparable results to in-house prepared water (Sartorius Arium purified water Type 1 and Type 3) and that 2) using filters (Sartorius Minisart RC, SRP, NY & NY Plus, all 0.2 µm) yielded comparable results to unfiltered specimens under the same mobile phase conditions.

Heavy Metals Testing in Cannabis

September 16, 2019

This application note demonstrates a simple way to test the heavy metals in cannabis using PERSEE’s PF7 heavy metal analyzer. Compare it with ICP/ICP-MS: you still can achieve lower detection limit, better repeatability with significant lower running cost, and simpler operation.

Analytical Data Confidence Is Highest with Commercially Prepared CRM Mixtures

September 16, 2019

High-quality reference materials with established metrological traceability are an essential element for measurement accuracy during routine analytical checks as well as for cannabis product quality testing and profiling. We compare two different methods using CRMs to generate a standard for the quantitation of 10 prevalent phytocannabinoids against a calibration curve to show that premade multicomponent CRM mixtures improve quantitation accuracy.