App Notes

Application Note: Preparation of Standards - Easier than ever

September 25, 2019

Improving your HPLC Sample Preparation is easier than ever with the Sartorius Cubis® MSA Dosing System. The Cubis MSA individual system with Q-App software automatically corrects the solvent volume to the appropriate value, eliminating the need to reach the precise target weight. At the end of the procedure, the weight of the compound(s) and solvents is used to calculate the verified concentration(s) and the verified volume of the standard prepared.

Application Note: Recovery & Adsorption of Therapeutic Proteins

September 25, 2019

We tested the novel filter device Claristep® for the preparation of protein samples containing one of four different target molecules: an anti-malaria vaccine candidate, aviscuminum, interferon alfa-2B, or monoclonal antibody (mAb) P2G12. In comparison to the standard protocols for sample preparation prior to analysis by either reversed-phase or SEC HPLC, the Claristep® filter did not exhibit a significant difference in protein concentration if the target molecules were present with at least 1.0 g L-1. A significantly reduced product concentration was observed in the Claristep® filtered samples compared to the standard protocol for mAb P2G12 if the concentrations were lower. The protein adsorption to the filter material was well described by a Langmuir adsorption isotherm. Additionally, the Claristep® filters facilitated a rapid sample preparation with minimal volume losses.

Poster: Analysis of Aqueous Mobile Phase Sample Prep & Filtration

September 25, 2019

The two-fold objective of this study was to 1) demonstrate that aqueous mobile phase preparations from purchased lab grade bottled water yielded comparable results to in-house prepared water (Sartorius Arium purified water Type 1 and Type 3) and that 2) using filters (Sartorius Minisart RC, SRP, NY & NY Plus, all 0.2 µm) yielded comparable results to unfiltered specimens under the same mobile phase conditions.

Heavy Metals Testing in Cannabis

September 16, 2019

This application note demonstrates a simple way to test the heavy metals in cannabis using PERSEE’s PF7 heavy metal analyzer. Compare it with ICP/ICP-MS: you still can achieve lower detection limit, better repeatability with significant lower running cost, and simpler operation.

Analytical Data Confidence Is Highest with Commercially Prepared CRM Mixtures

September 16, 2019

High-quality reference materials with established metrological traceability are an essential element for measurement accuracy during routine analytical checks as well as for cannabis product quality testing and profiling. We compare two different methods using CRMs to generate a standard for the quantitation of 10 prevalent phytocannabinoids against a calibration curve to show that premade multicomponent CRM mixtures improve quantitation accuracy.

Microwave-Assisted Digestion of Various Cannabis Products with Pressure-Activated Venting

September 16, 2019

This application report demonstrates the suitability of HVT vessels with SMART VENT Technology for convenient and reliable digestion of cannabis-based products prior to spectroscopic analysis.

Microwave Digestion and Elemental Analysis of Cannabis and Hemp Products

September 13, 2019

This application note demonstrates the CEM MARS 6 for microwave digestion of mixed cannabis and hemp containing products in a single run and elemental analysis of these products.

CRM Mixtures Improve Quantitation Accuracy

September 13, 2019

Pre-made, multicomponent CRM mixtures save time and consumable cost in the preparation of standard curves and improve quantitation accuracy.

Analysis of Cannabinoids in Hemp

September 13, 2019

This application note outlines a QuEChERS method for the analysis of hemp for three cannabinoids. Sample purification is carried out using UCT’s new cleanup product SpinFiltr™.