Chromatography Techniques

Patricia Atkins is a Senior Applications Scientist with SPEX CertiPrep and a member of both the AOAC and ASTM committees for cannabis.

Articles

Let’s investigate the differences between the commercially available detector in terms of sample type, size, and selectivity to understand which detectors fit into the most common types of cannabis GC analyses.

Looking with Light: Understanding Gas Chromatography, Part IV: Detectors

This article investigates the chiral method development screening for the enantiomeric separation of synthetic CBD on polysaccharide-based chiral stationary phases with both normal-phase and reversed phase mobile phases, using HPLC and UHPLC.

The Chiral Separation of the (+) and (-) Enantiomers of Cannabidiol

Let’s try to understand the functioning and chemistry of GC columns and how to change and manipulate the resolution equation and column chemistry to maximize our own analyses.

Looking with Light: Understanding Gas Chromatography, Part III: Columns and Resolution

This study focuses on a step-by-step optimization of commercial scale preparative HPLC purification of cannabinoids, specifically cannabidiol, starting with stationary reversed-phase screening, followed by method optimization.

Development, Optimization, and Process Performance for Preparative Chromatographic Purification of Cannabinoids

It is demonstrated in this paper that cell viability can be used to determine dose and response effects that are important in understanding toxicities and to detect differences in similar hemp products.

Human Cells Can Be Used to Study Cannabinoid Dosage and Inflammatory Cytokine Responses

Brian C. Smith, PhD, is Founder, CEO, and Chief Technical Officer of Big Sur Scientific. He is the inventor of the BSS series of patented mid-infrared based cannabis analyzers. Dr. Smith has done pioneering research and published numerous peer-reviewed papers on the application of mid-infrared spectroscopy to cannabis analysis, and sits on the editorial board of Cannabis Science and Technology. He has worked as a laboratory director for a cannabis extractor, as an analytical chemist for Waters Associates and PerkinElmer, and as an analytical instrument salesperson. He has more than 30 years of experience in chemical analysis and has written three books on the subject. Dr. Smith earned his PhD on physical chemistry from Dartmouth College. Direct correspondence to: 

In part I of this series on statistical concepts related to cannabis analysis, we introduce the normal distribution, standard deviation, and how to calculate precision and accuracy.

Statistics for Cannabis Analysis, Part I: Standard Deviation and Its Relationship to Accuracy and Precision

What is the overall scope of spectroscopy and how can it be used as a tool to improve analyses?

Examining Energy and Light: Understanding Spectroscopy and Spectrophotometry

We finish looking at liquid chromatography with a deeper examination of the mechanics, physics, and chemistry of different spectrometers that are used in analytical laboratories.

Looking with Light: Understanding Spectrometry

A number of misconceptions people have about how to test, what to test, and when to test in the cannabis industry are dispelled.

Dispelling Cannabis Analysis Myths, Part I

A systematic literature review to identify and compare analytical methods used for the determination of cannabinoids in different matrices using LC-based systems.