In Memoriam: Anthony Macherone

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It is with great sadness that we acknowledge the passing of Anthony Macherone, PhD.

Anthony Macherone, PhD., an Analytical Chemist for Agilent Technologies, was a valued member of our Editorial Advisory Board and a highly experienced expert in the field of cannabis and hemp testing and analysis. He led a team of scientists in the development of analytical methodologies to support research, safety, and quality control for the emerging cannabis and hemp fields, and has written extensively on cannabis testing, contributing to several chapters to books about cannabis. His recent articles published in Cannabis Science and Technology include, “How Manufacturing, Regulations, and Sustainability Are Affecting the Cannabis and Hemp-Based Products Industry,” and “Fast Residual Solvents and Terpenes Analyses in Cannabis and Hemp Products,” co-authored with Jeffery S. Hollis and featured in our January/February 2024 issue.

Dr. Macherone was also a trusted advisor on the educational steering committee for the Cannabis Science Conference. He presented “LC–MS For Regulatory Testing: It’s About Time,” at our Fall 2022 conference and we were fortunate to have highlighted Dr. Macherone in an interview from the conference as well.

We greatly appreciate his contributions to our publications and to advancements in the cannabis industry. He will be missed by many.

A Selection of Dr. Macherone’s Work with Cannabis Science and Technology


Dr. Macherone on Cannabis Science Conference East Education and Future Growth Areas

Dr. Anthony Macherone of Agilent Technologies shares what he learned at Cannabis Science Conference East 2022.

Dr. Anthony Macherone Calls for More Comprehensive Pesticide Testing for Safer Consumer Cannabis Products

Dr. Anthony Macherone of Agilent Technologies discusses what he hopes to see change in the future regarding cannabis regulations and testing guidelines.


Challenges in Developing Cannabis Testing Methods

Dr. Anthony Macherone of Agilent Technologies discusses some of the unique challenges when developing testing methods for cannabis products.

Dr. Anthony Macherone Discusses LC-MS for Potency Testing at CSC East 2022

Dr. Anthony Macherone of Agilent Technologies shares some insights from his talk at Cannabis Science Conference East 2022.

Podcast Series

"a"-Musings from the Cannabis Corner

A six-part podcast series moderated by Dr. Macherone about cannabis testing and regulations in the Americas and globally.



The Stability of Acid Phytocannabinoids Using Electrospray Ionization LC–MS in Positive and Negative Modes

This study highlights a potential concern for the quantitation of acid phytocannabinoids.

A Brief Review of Derivatization Chemistries for the Analysis of Cannabinoids Using GC–MS

This article focuses on the use of GC–MS for the analysis of derivatized cannabinoids in hempseed oil matrix.


Up in Smoke: The Naked Truth for LC–MS/MS and GC–MS/MS Technologies for the Analysis of Certain Pesticides in Cannabis Flower

A study using LC-qTOF, GCqTOF, and GC–MS/MS to evaluate the selectivity of a model pesticide commonly found in regulatory target lists.

GC-TOF Discovery-Based Profiling of CBD Oil Pet Supplements

In this study, six different brands of CBD oil pet supplements were obtained and untargeted analyses using gas phase-time of flight mass spectrometry were performed.

Optimized Cannabis Microbial Testing: Combined Use of Extraction Methods and Pathogen Detection Tests Using Quantitative Polymerase Chain Reaction

This review introduces a quantitative polymerase chain reaction (qPCR) assay and compares its performance to culture-based methods.


Liquid Chromatography– Time-of-Flight Mass Spectrometry for Cannabinoid Profiling and Quantitation in Hemp Oil Extracts

The liquid chromatography–time-of-flight mass spectrometry (LC–TOF-MS) method presented herein allows for the accurate, precise, and robust speciation, profiling, and quantification of cannabinoids in hemp oil extracts and commercial cannabinoid products for research and development laboratories.

A Comprehensive Approach to Pesticide Residue Analysis in Cannabis

Here we present a straightforward acetonitrile extraction using a solid-phase extraction (SPE) cartridge and targeted dispersive solid-phase extraction (dSPE) cleanup. The final dilute extract is analyzed with both gas chromatography–tandem mass spectrometry (GC–MS/MS) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for a comprehensive target list (200+ compounds) that encompasses those identified on individual U.S. state lists.