GC-TOF Discovery-Based Profiling of CBD Oil Pet Supplements

December 18, 2019
Abstract / Synopsis: 

Cannabidiol (CBD) oils manufactured specifically for pets is a rapidly growing market. Many pet owners are supplementing their pets’ diet with products derived legally from hemp. In this study, we obtained five lots of six different brands of CBD oil pet supplements and performed untargeted analyses using gas phase-time of flight mass spectrometry. Chemical features of the raw data were extracted and annotated, and differential statistical analysis using commercially available bioinformatics software was used to examine each product.

With the recent phenomenon of legalization of cannabis, hemp, and cannabinoid products for both medicinal and adult recreational use in many U.S. states and Canada, many pet owners are feeding cannabidiol (CBD) oils derived from hemp to their pets. CBD is a nonpsychoactive, natural phytocannabinoid native to Cannabis spp. and is commercially available. A quick search of the web yields hundreds of products specifically made for pets. In this study, we used a nontargeted chemical analysis approach to evaluate the chemical profiles of commercially available CBD oils manufactured for pet consumption. We purchased five bottles of each product to investigate batch diversity and different lots of each product. It’s important to note that this workflow can be equally applied to plant material or other cannabinoid products for large scale cannabinomics, terpenomics, metabolomics, or other “big-data” studies.

Materials and Methods

Chemicals and Standards
Five bottles of six commercially available CBD oil pet supplements (n = 30) were purchased online. High purity dichloromethane (DCM) was used to solubilize and dilute the oil samples. A C5–C30 alkane mixture was used to define retention indices, and an isotopically labeled polyaromatic hydrocarbon (PAH) mixture containing naphthalene-d8, acenaphthalene-d10, chrysene-d12, and perylene-d12 was used as internal standards.

Instrument Hardware and Software
An Agilent 7890/7250 gas chromatography-quadrupole time-of-flight (GC-QTOF) system was used for all analyses. MassHunter software was used for acquisition and qualitative analysis. Mass Hunter Unknowns Analysis software was used for feature finding. Mass Profiler Professional software was used for differential statistical analysis. See Tables I and II for GC and QTOF parameters, respectively.

Table 1

Table 2

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  2. ©2014 copyright by the U.S. Secretary of Commerce on behalf of the United States of America.
  3. R. Andrews and S. Paterson, J. Analyt. Tox. 36, 61–65 DOI:10.1093/jat/bkr017 (2012).

About the Authors

Matthew Curtis and Sue D’Antonio are with Agilent Technologies in Santa Clara, California. Anthony Macherone is with Agilent Technologies and The Johns Hopkins University School of Medicine in Baltimore, Maryland. Direct correspondence to: [email protected]

How to Cite this Article

M. Curtis, S. D’Antonio, and A. Macherone, Cannabis Science and Technology 2(6), 48-52 (2019).