Chromatography Techniques

Liquid Chromatography– Time-of-Flight Mass Spectrometry for Cannabinoid Profiling and Quantitation in Hemp Oil Extracts

February 28, 2018

A primary impediment to cannabinoid research is the fact that materials possessing psychoactive Δ9-tetrahydrocannabinol (THC) are considered Schedule I drugs as defined in the U.S. Controlled Substances Act. An alternative source of cannabinoids may be found in hemp oil extracts. Hemp contains a low percentage of THC by weight but relatively high amounts of nonpsychoactive cannabinoids. The liquid chromatography–time-of-flight mass spectrometry (LC–TOF-MS) method presented herein allows for the accurate, precise, and robust speciation, profiling, and quantification of cannabinoids in hemp oil extracts and commercial cannabinoid products for research and development laboratories. The method was determined to chromatographically separate 11 cannabinoids including differentiation of Δ8-tetrahdrocannabinol and THC with excellent linear dynamic range, specificity, and sensitivity.

Analysis of Terpenes in Cannabis Using Headspace Solid-Phase Microextraction and GC–MS

February 28, 2018

Headspace SPME combined with GC–MS for the qualitative and quantitative analysis of terpenes in cannabis offers several advantages compared to other methods. It does not require the use of organic solvents, does not coextract matrix, and provides additional means of peak identification and purity using spectral data. It is also a nondestructive method.

Using Compact Mass Spectrometry for Detection and Quantification of Cannabis-Related Compounds

February 27, 2018

Compact mass spectrometry, in combination with suitable sample introduction techniques—such as the atmospheric solids analysis probe, thin-layer chromatography, and classical liquid chromatography techniques—can be used effectively for the detection and quantification of cannabinoids and pesticides in cannabis-related material and contraband.

Trends in Cannabis Analysis

February 27, 2018

An interview with Jeff Dahl, an applications scientist at Shimadzu Scientific Instruments; Joe Konschnik, a business development manager for the food and beverage market at Restek Corporation; Andre Santos, the Americas market development manager at Agilent Technologies; Alicia D. Stell, the CEM product development lab manager; and Xiaoyan Wang, a research scientist at UCT

Rapid Determination of 24 Synthetic and Natural Cannabinoids for LC–MS-MS Screening in Natural Products and Drug Inspection Applications

February 26, 2018

Other techniques for monitoring cannabinoids, such as GC–MS and LC–MS, are limited to screening known species and are therefore always one step behind the designer drug market—where new, previously unknown variations of analogs of cannabinoids are constantly being synthesized. The method presented here addresses this problem by providing accurate masses for all detected species, thus allowing postanalysis identification of initially untargeted compounds.

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